%0 Journal Article
%3 lobo.pdf
%@mirrorrepository sid.inpe.br/mtc-m19@80/2009/08.21.17.02.53
%4 sid.inpe.br/mtc-m19@80/2010/01.12.12.55
%8 Jan.
%@issn 0008-6223
%A Lobo, Anderson de Oliveira,
%A Corat, M. A. F.,
%A Antunes, E. F.,
%A Palma, M. B. S.,
%A Pacheco-Soaresd,
%A Garcia, E. E.,
%A Corat, E. J.,
%@secondarytype PRE PI
%B Carbon
%D 2010
%F self-archiving-INPE-MCTIC-GOV-BR
%@secondarykey INPE--PRE/
%K Multiwalled carbon nanotubes (MWCN) -  Adhesion  -  Biocompatibility  -  Carbon films  -  Carbon nanotubes  -  Cell adhesion  -  Cell culture  -  Cell growth  -  Cell membranes  -  Cell proliferation  -  Growth kinetics   -  High resolution electron microscopy  -  Inductively coupled plasma  -  Metal refining  -  Microwaves  -  Nickel  -  Optical emission spectroscopy  -  Plasma deposition  -  Scanning electron microscopy  -  Titanium.
%@archivingpolicy denypublisher denyfinaldraft24
%N 1
%P 245-254
%@secondarymark A2_ASTRONOMIA_/_FÍSICA A1_ENGENHARIAS_II A1_ENGENHARIAS_III B1_ENGENHARIAS_IV C_ENSINO_DE_CIÊNCIAS_E_MATEMATICA A1_INTERDISCIPLINAR A2_MATERIAIS A1_QUÍMICA
%T An evaluation of cell proliferation and adhesion on vertically-aligned multi-walled carbon nanotube films
%V 48
%X Biocompatibility tests were performed on vertically-aligned multi-walled carbon nanotube (MWCNT) films produced by microwave plasma chemical vapor deposition on titanium substrates with iron (Fe) and nickel (Ni) as catalysts. The cell adhesion and morphology of L-929 mouse fibroblast cells were studied by high resolution scanning electron microscopy, after up to 7 days incubation periods. Cell viability and proliferation were evaluated by two in vitro tests: (1) 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (MTT), and (2) lactate dehydrogenase (LDH) assays. Low level of bioavailable Fe and Ni was determined by inductively coupled plasma optical emission spectrometry. Neither functionalization nor purification of MWCNT films was necessary to obtain good response to the biocompatibility tests. Efficient cell growth and non-toxicity suggest the use MWCNTs in tissue regeneration. The MWCNT films stimulated the cell growth, showing a proliferation 20% higher than on Ti.
%@area FISMAT
%@e-mailaddress marciana@sid.inpe.br
%@documentstage not transferred
%@group
%@group
%@group LAS-CTE-INPE-MCT-BR
%@group
%@group
%@group
%@group LAS-CTE-INPE-MCT-BR
%@dissemination WEBSCI; PORTALCAPES.
%@usergroup administrator
%@usergroup banon
%@usergroup marciana@sid.inpe.br
%@nexthigherunit 8JMKD3MGPCW/3ESR3H2
%@affiliation
%@affiliation Centro Multidisciplinar para Investigacao Biologica Area Ciencia em Animais de Laboratorio - CEMIB, Universidade Estadual de Campinas (UNICAMP)
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@affiliation Laboratório de Dinâmica de Compartimentos Celulares, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Sao Jose dos Campos, SP
%@affiliation Laboratório de Dinâmica de Compartimentos Celulares, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Sao Jose dos Campos, SP
%@affiliation Departamento de Química, Universidade Estadual de Maringá, Maringá/Pr, CEP: 87020-090, Brazil
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@versiontype publisher
%@holdercode {isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S}
%@doi 10.1016/j.carbon.2009.09.012
%2 sid.inpe.br/mtc-m19@80/2010/01.12.12.55.45